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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
21/06/2023 |
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Actualizado : |
21/06/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
RODRIGUEZ, N.E.; BEYHAUT, E.; SICARDI, M.; RODRÍGUEZ-BLANCO, A. |
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Afiliación : |
N. E. RODRIGUEZ RODRIGUEZ, Depto. de Biología Vegetal, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay; ELENA BEYHAUT GUTIERREZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; M. SICARDI, Instituto de Ecología y Ciencias Ambientales, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay; A. RODRÍGUEZ-BLANCO, Depto. de Biología Vegetal, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay. |
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Título : |
Bioprospection of naturalized soybean-nodulating Bradyrhizobium strains in Uruguayan soils: a genetic and symbiotic approach. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Environmental Sustainability. 2023, volume 6, pages 161-171. https://doi.org/10.1007/s42398-022-00258-1 |
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ISSN : |
2523-8922 (electronic). |
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DOI : |
10.1007/s42398-022-00258-1 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 31 May 2022; Revised 29 September 2022; Accepted 6 December 2022; Published online 5 January 2023. -- Correspondence: A. Rodríguez-Blanco. Depto. de Biología Vegetal, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay, email: andrearb@fagro.edu.uy -- |
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Contenido : |
ABSTRACT.- Soybean (Glycine max (L.) Merr.), an economically relevant crop, establishes a symbiotic association with rhizobia to obtain nitrogen (N2) from the air by biological nitrogen fixation with important benefits. In Uruguay inoculants formulated with two strains, Bradyrhizobium elkanii U1301 and U1302, are recommended since 1984. Besides that, the study of native-naturalized rhizobia populations is relevant because these strains could compete with applied inoculants and may present a better symbiotic efficiency. The aim of this work was to study, genetically and symbiotically, naturalized soybean nodulating rhizobia isolated from Uruguayan soils. A collection of ten naturalized rhizobia was studied and compared with Uruguay's commercial strains and neighbouring countries (B. elkanii U1301 and U1302, Bradyrhizobium japonicum E109 and Bradyrhizobium diazoefficiens SEMIA5080). Using a multilocus sequence analysis (MLSA) (16S rRNA, atpD, gyrB and rpoB genes), five naturalized strains were identified as B. elkanii and four as B. japonicum. The other naturalized strain UYS-CA02 is suggested to belong to Bradyrhizobium ferriligni, considering a second MLSA with 16S rRNA, gyrB, rpoB, dnaK and recA genes. Analysis of symbiotic genes (nodY/K and nifH) indicates that strains U1301 and U1302 may have transferred these genes horizontally to strain UYS-CA02 or its ancestor. Symbiotic efficiency was evaluated in axenic conditions, in which shoot dry weight, total nitrogen in shoots, number of nodules and nodules dry weight, were determined. In that assay, the U1301:U1302 blend outstood in front of other commercial strains. Multivariate analysis of symbiotic efficiency data shows a better performance of B. elkanii-like strains than B. japonicum-like ones. The Author(s) under exclusive licence to Society for Environmental Sustainability 2023. MenosABSTRACT.- Soybean (Glycine max (L.) Merr.), an economically relevant crop, establishes a symbiotic association with rhizobia to obtain nitrogen (N2) from the air by biological nitrogen fixation with important benefits. In Uruguay inoculants formulated with two strains, Bradyrhizobium elkanii U1301 and U1302, are recommended since 1984. Besides that, the study of native-naturalized rhizobia populations is relevant because these strains could compete with applied inoculants and may present a better symbiotic efficiency. The aim of this work was to study, genetically and symbiotically, naturalized soybean nodulating rhizobia isolated from Uruguayan soils. A collection of ten naturalized rhizobia was studied and compared with Uruguay's commercial strains and neighbouring countries (B. elkanii U1301 and U1302, Bradyrhizobium japonicum E109 and Bradyrhizobium diazoefficiens SEMIA5080). Using a multilocus sequence analysis (MLSA) (16S rRNA, atpD, gyrB and rpoB genes), five naturalized strains were identified as B. elkanii and four as B. japonicum. The other naturalized strain UYS-CA02 is suggested to belong to Bradyrhizobium ferriligni, considering a second MLSA with 16S rRNA, gyrB, rpoB, dnaK and recA genes. Analysis of symbiotic genes (nodY/K and nifH) indicates that strains U1301 and U1302 may have transferred these genes horizontally to strain UYS-CA02 or its ancestor. Symbiotic efficiency was evaluated in axenic conditions, in which shoot dry weight, total nitrogen in shoots,... Presentar Todo |
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Palabras claves : |
Biological nitrogen fixation; Bradyrhizobium; Glycine max; MLSA; Phylogeny; PLATAFORMA DE BIOINSUMOS - INIA; Rhizobia. |
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Asunto categoría : |
P01 Conservación de la naturaleza y recursos de La tierra |
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Marc : |
LEADER 03065naa a2200277 a 4500
001 1064205
005 2023-06-21
008 2023 bl uuuu u00u1 u #d
022 $a2523-8922 (electronic).
024 7 $a10.1007/s42398-022-00258-1$2DOI
100 1 $aRODRIGUEZ, N.E.
245 $aBioprospection of naturalized soybean-nodulating Bradyrhizobium strains in Uruguayan soils$ba genetic and symbiotic approach.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 31 May 2022; Revised 29 September 2022; Accepted 6 December 2022; Published online 5 January 2023. -- Correspondence: A. Rodríguez-Blanco. Depto. de Biología Vegetal, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay, email: andrearb@fagro.edu.uy --
520 $aABSTRACT.- Soybean (Glycine max (L.) Merr.), an economically relevant crop, establishes a symbiotic association with rhizobia to obtain nitrogen (N2) from the air by biological nitrogen fixation with important benefits. In Uruguay inoculants formulated with two strains, Bradyrhizobium elkanii U1301 and U1302, are recommended since 1984. Besides that, the study of native-naturalized rhizobia populations is relevant because these strains could compete with applied inoculants and may present a better symbiotic efficiency. The aim of this work was to study, genetically and symbiotically, naturalized soybean nodulating rhizobia isolated from Uruguayan soils. A collection of ten naturalized rhizobia was studied and compared with Uruguay's commercial strains and neighbouring countries (B. elkanii U1301 and U1302, Bradyrhizobium japonicum E109 and Bradyrhizobium diazoefficiens SEMIA5080). Using a multilocus sequence analysis (MLSA) (16S rRNA, atpD, gyrB and rpoB genes), five naturalized strains were identified as B. elkanii and four as B. japonicum. The other naturalized strain UYS-CA02 is suggested to belong to Bradyrhizobium ferriligni, considering a second MLSA with 16S rRNA, gyrB, rpoB, dnaK and recA genes. Analysis of symbiotic genes (nodY/K and nifH) indicates that strains U1301 and U1302 may have transferred these genes horizontally to strain UYS-CA02 or its ancestor. Symbiotic efficiency was evaluated in axenic conditions, in which shoot dry weight, total nitrogen in shoots, number of nodules and nodules dry weight, were determined. In that assay, the U1301:U1302 blend outstood in front of other commercial strains. Multivariate analysis of symbiotic efficiency data shows a better performance of B. elkanii-like strains than B. japonicum-like ones. The Author(s) under exclusive licence to Society for Environmental Sustainability 2023.
653 $aBiological nitrogen fixation
653 $aBradyrhizobium
653 $aGlycine max
653 $aMLSA
653 $aPhylogeny
653 $aPLATAFORMA DE BIOINSUMOS - INIA
653 $aRhizobia
700 1 $aBEYHAUT, E.
700 1 $aSICARDI, M.
700 1 $aRODRÍGUEZ-BLANCO, A.
773 $tEnvironmental Sustainability. 2023, volume 6, pages 161-171. https://doi.org/10.1007/s42398-022-00258-1
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
02/10/2017 |
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Actualizado : |
02/10/2017 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
FRIONI, N.; ROVERE, G.; AGUILAR, I.; URIOSTE, J. |
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Afiliación : |
NICOLÁS FRIONI, Universidad de la República (UdelaR)/ Facultad de Agronomía (UY); Animal Breeding and Genetics Group, Department of Animal Sciences, Georg-August-University (DE); GABRIEL ROVERE, Universidad de la República (UdelaR)/ Facultad de Agronomía (UY); Department of Molecular Biology and Genetics, Centre for Quantitative Genetics and Genomics, Aarhus University (DK); IGNACIO AGUILAR GARCIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JORGE I. URIOSTE, Universidad de la República (UdelaR)/ Facultad de Agronomía (UY). |
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Título : |
Genetic parameters and correlations between days open and production traits across lactations in pasture based dairy production systems. |
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Fecha de publicación : |
2017 |
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Fuente / Imprenta : |
Livestock Science, 2017, 204:104-109. |
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ISSN : |
1871-1413 |
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DOI : |
10.1016/j.livsci.2017.08.018 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 14 November 2016; Received in revised form 20 June 2017; Accepted 23 August 2017. |
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Contenido : |
ABSTRACT.
The aim of this study was to estimate the additive genetic correlations, heritabilities and repeatabilities of days open (DO), milk (MY), fat (FY) and protein yields (PY), using data from pasture based dairy systems of Uruguay, and to explore whether DO should be interpreted as a different trait across lactations or as a trait with repeated measures. The database contained 500, 412 and 294 thousand records of first, second and third lactation, respectively. Cows were offspring of 7747 sires. Fertility records lower and upper limits were 42 and 250 days, respectively. In a first approach (Mdiff) we estimated variance components and covariances over lactations, assuming that the traits were different at each lactation. In a second approach (Mrep) estimations were carried out considering each trait as a repeated measure along lactations. In Mdiff, DO with a production trait was analyzed considering each lactation as a different trait. Three six-variate linear models were analyzed (DO-MY, DO-FY, DO-PY, and lactations first to third). In the Mrep procedure, DO, MY, FY and PY were analyzed together with a multiple trait repeatability model. For all models, the fixed effects were herd-year-season and lactationage classes. Animal and the permanent environment effect were included as random effects. The additive genetic correlations between DO and yield traits by Mdiff were between +0.39 and +0.78; by Mrep, they ranged from +0.44 to +0.55. Heritabilities of DO by Mdiff were between 0.04 and 0.06 and 0.05 by Mrep. The additive genetic correlations of DO between lactations ranged from +0.76 to +0.91. Heritabilities of MY, FY and PY were 0.23, 0.21 and 0.21, respectively. Repeatabilities obtained were 0.10, 0.49, 0.47 and 0.49 for DO, MY, FY and PY, respectively. We concluded that the heritability of DO was low but enough to consider the trait in selection programs. We confirmed unfavorable additive genetic correlations between DO and yield traits for the Uruguayan pasture systems, which supports the importance of considering fertility in selection programs to reduce or avoid a decline in reproduction. The additive genetic correlations of DO between lactations were high, suggesting that a plausible model should consider DO records of a given animal as repeated measures.
© 2017 Elsevier B.V. All rights reserved. MenosABSTRACT.
The aim of this study was to estimate the additive genetic correlations, heritabilities and repeatabilities of days open (DO), milk (MY), fat (FY) and protein yields (PY), using data from pasture based dairy systems of Uruguay, and to explore whether DO should be interpreted as a different trait across lactations or as a trait with repeated measures. The database contained 500, 412 and 294 thousand records of first, second and third lactation, respectively. Cows were offspring of 7747 sires. Fertility records lower and upper limits were 42 and 250 days, respectively. In a first approach (Mdiff) we estimated variance components and covariances over lactations, assuming that the traits were different at each lactation. In a second approach (Mrep) estimations were carried out considering each trait as a repeated measure along lactations. In Mdiff, DO with a production trait was analyzed considering each lactation as a different trait. Three six-variate linear models were analyzed (DO-MY, DO-FY, DO-PY, and lactations first to third). In the Mrep procedure, DO, MY, FY and PY were analyzed together with a multiple trait repeatability model. For all models, the fixed effects were herd-year-season and lactationage classes. Animal and the permanent environment effect were included as random effects. The additive genetic correlations between DO and yield traits by Mdiff were between +0.39 and +0.78; by Mrep, they ranged from +0.44 to +0.55. Heritabilities of DO by Mdiff were... Presentar Todo |
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Palabras claves : |
DAIRY PRODUCTION; GENETIC CORRELATION; HERITABILITY; HOLSTEIN; REPRODUCTIVE PERFORMANCE. |
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Thesagro : |
GANADO LECHERO; RENDIMIENTO LECHERO. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 03282naa a2200277 a 4500
001 1057620
005 2017-10-02
008 2017 bl uuuu u00u1 u #d
022 $a1871-1413
024 7 $a10.1016/j.livsci.2017.08.018$2DOI
100 1 $aFRIONI, N.
245 $aGenetic parameters and correlations between days open and production traits across lactations in pasture based dairy production systems.$h[electronic resource]
260 $c2017
500 $aArticle history: Received 14 November 2016; Received in revised form 20 June 2017; Accepted 23 August 2017.
520 $aABSTRACT. The aim of this study was to estimate the additive genetic correlations, heritabilities and repeatabilities of days open (DO), milk (MY), fat (FY) and protein yields (PY), using data from pasture based dairy systems of Uruguay, and to explore whether DO should be interpreted as a different trait across lactations or as a trait with repeated measures. The database contained 500, 412 and 294 thousand records of first, second and third lactation, respectively. Cows were offspring of 7747 sires. Fertility records lower and upper limits were 42 and 250 days, respectively. In a first approach (Mdiff) we estimated variance components and covariances over lactations, assuming that the traits were different at each lactation. In a second approach (Mrep) estimations were carried out considering each trait as a repeated measure along lactations. In Mdiff, DO with a production trait was analyzed considering each lactation as a different trait. Three six-variate linear models were analyzed (DO-MY, DO-FY, DO-PY, and lactations first to third). In the Mrep procedure, DO, MY, FY and PY were analyzed together with a multiple trait repeatability model. For all models, the fixed effects were herd-year-season and lactationage classes. Animal and the permanent environment effect were included as random effects. The additive genetic correlations between DO and yield traits by Mdiff were between +0.39 and +0.78; by Mrep, they ranged from +0.44 to +0.55. Heritabilities of DO by Mdiff were between 0.04 and 0.06 and 0.05 by Mrep. The additive genetic correlations of DO between lactations ranged from +0.76 to +0.91. Heritabilities of MY, FY and PY were 0.23, 0.21 and 0.21, respectively. Repeatabilities obtained were 0.10, 0.49, 0.47 and 0.49 for DO, MY, FY and PY, respectively. We concluded that the heritability of DO was low but enough to consider the trait in selection programs. We confirmed unfavorable additive genetic correlations between DO and yield traits for the Uruguayan pasture systems, which supports the importance of considering fertility in selection programs to reduce or avoid a decline in reproduction. The additive genetic correlations of DO between lactations were high, suggesting that a plausible model should consider DO records of a given animal as repeated measures. © 2017 Elsevier B.V. All rights reserved.
650 $aGANADO LECHERO
650 $aRENDIMIENTO LECHERO
653 $aDAIRY PRODUCTION
653 $aGENETIC CORRELATION
653 $aHERITABILITY
653 $aHOLSTEIN
653 $aREPRODUCTIVE PERFORMANCE
700 1 $aROVERE, G.
700 1 $aAGUILAR, I.
700 1 $aURIOSTE, J.
773 $tLivestock Science, 2017, 204:104-109.
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